Plant tissue culture The new magical mix of Success
The plant tissue culture becomes a very vital interest for all who need to learn about this culture. This Culture can be defined as maintenance technique by growing the cells, tissues or organs. this is especially on the artificial media in containers which have to be suitable and under controlled environmental conditions. This was the Most familiar Defenition for the Plant tissue culture.
This post Written by Eng. Ahmed AbdEllah
The Plant tissue culture The new magical mix of Success:
This is your detailed guide to know all details about the following headlines:
- Plant tissue culture uses
- Making mutations
- plant tissue culture types
- Conditions for successful plant tissue culture.
- Major salts
- Minor salts
- Vitamins & hormones.
- Transfer room
- Steps of transferring.
- multiplication and shooting.
- transfer room Components
- Growth Room
Keep Reading and Feel free to leave any comment you need to write the post
Plant tissue culture uses :
There is so many plant tissue culture uses I will give you Some important details about it.
By cloning huge numbers of plants which have the same genetic traits in a very short time. In Cloning, we select good plant and reproduce many plants from it. Also, It is useful for production plants has good qualities. The Multiplication is useful too for producing large numbers of endangered plants.
Plant breeding needs many years to get plants has specific qualities. But On the other hand, with plant tissue culture it will be too short.
producing ornamental plants has new colors or shapes by adding mutagens like T4D to the medium.
Plant tissue culture types :
There are a lot of types of the plant tissue culture types which are the following:
- Seed culture
- Embryo culture
- Callus culture
- Organs culture
- Protoplast culture
This was some of the most familiar types
Conditions for successful plant tissue culture :
Select the suitable part of the plant (Explant)
Because every kind of plant has a specific part which can produce an embryo by tissue culture. This explant can be buds, parts of the leaf, parts of roots or parts of stem).
Suitable plant growth medium
To stimulate the explant to produce embryo you must prepare a specific medium which contains specific quantities of major salts, minor salts, hormones, vitamins and sometimes hardener materials like Agar. Every plant needs specific growth medium and every step of plant tissue culture for the same plant need specific growth medium. Preparing growth medium is the most important thing in plant tissue culture, it’s the secret of success. The most common growth medium called (m.s) which discovered by Murashige and Skoog which consisting of:
Major salts :
There are some major salts
- NH4NO3 (1.650 mg/l)
- 2H2O (440 mg/l)
- 7H2O (370mg/l)
- KNO3 (1.900 mg/l)
- KH2PO4 (170 mg/l)
Minor salts :
- H3BO3 (6.54 mg/l)
- 6H2O (0.025 mg/l)
- 7H2O (27.8 mg/l)
- 4H2O (22.3 mg/l)
- KI (0.83 mg/l)
- 2H2O (0.25 mg/l)
- 7H2O (8.6 mg/l)
- NaFe-EDTA (5ml/l of stock solution containing 5.57g FeSO4.7H2O & 7.45g Na2-EDTA / liter of water
- 5H2O 0.025 mg/l
Vitamins & hormones :
Here we will talk about the basics of the important Vitamins & hormones
- Myo-Inositol (100mg/l)
- Nicotinic Acid (0.5mg/l)
- HCl (0.1mg/l)
- HCl (0.5mg/l)
- Indole Acetic Acid (1-30mg/l)
- Lactalbumin Hydrolysate (1g/l)
- Glycine (2mg/l)
- Kinetin (0.04-10mg/l
This was the most familiar items if you have any further items feel free to leave them on the comment side
You must avoid contamination in each part of your lap
Departments of plant tissue culture lap :
The kitchen is the place we prepare the growth medium in and it consists of
- Water distillation device
- 4 digit scale
- Magnetic stirrer
- Jars have covers don,t allow bacteria entry
The department we separate the explant and move it to another media jars or in vitro in
Steps of transferring:
by Selecting and cutting the suitable explant and wash it with water and sterilize it with ethanol 70% vol. then put it in suitable medium then transfer it to growth room. After few days the explant will make embryos or callus
- Multiplication and shooting
bye transfer the explant to new media to stimulate it to bud and making stems and leaves then we can separate every two or three plants and put it in the new medium to stimulate it to make new plants then put it in growth room again (we can do this step many times)
by separating plants (each plant is alone) and put it in a medium containing oxine to stimulate the plant to make roots.
- The transfer room Components:
This room consists of all of the following items :
- Tissue culture hood ( Laminar flow )
consists of glass cabins opened from the front and from back there is Hepa filter (which the unicellular organism can,t pass from its holes).
this HEPA filter connected to air flow compressor to make an air curtain which don,t allow the unicellular organism enter the cabins.
- Transfer tools
(Scalpel and forceps)
- Tools sterilizer
- Growth room
The department we keep jars which come from transfer room under best condition (heat & light units) to grow up and prepare it for next step.
We get small plants and we have to move it greenhouse for acclimation then move it to the farm.